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one step blue protein gel stain  (Biotium)


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    Structured Review

    Biotium one step blue protein gel stain
    One Step Blue Protein Gel Stain, supplied by Biotium, used in various techniques. Bioz Stars score: 95/100, based on 69 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/one+step+blue+protein+gel+stain/One-Step+Blue+Protein+Gel+Stain/pmc13106227-278-7-12
    Average 95 stars, based on 69 article reviews
    one step blue protein gel stain - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Electrophoresis:

    Article Title: Comparative Effects of Dry and Wet Aging on Quality, Flavor Development, and Metabolomic Profiles in Grouper Fillets
    Article Snippet: .. Following electrophoresis, the gels were stained with One‐Step Blue protein gel stain (Biotium, Inc., Fremont, CA, USA) for 1 h and destained in distilled water for ~20 h. ..

    Staining:

    Article Title: Comparative Effects of Dry and Wet Aging on Quality, Flavor Development, and Metabolomic Profiles in Grouper Fillets
    Article Snippet: .. Following electrophoresis, the gels were stained with One‐Step Blue protein gel stain (Biotium, Inc., Fremont, CA, USA) for 1 h and destained in distilled water for ~20 h. ..

    Article Title: Fc receptor dependent and independent mechanisms of antibody-mediated enhancement of immune responses
    Article Snippet: Briefly, antibodies were incubated with NuPAGETM Sample Reducing Agent (Invitrogen NP0009) and NuPAGETM LDS sample buffer (Invitrogen NP0007), heated shortly at 95°C and run on NuPAGETM Bis-Tris Mini Protein Gels (Thermo Fisher NP0321Box). .. Protein gels were stained using One-Step Blue® Protein Gel Stain (Biotium 21003-1L) and subsequently imaged using an AmershamTM ImageQuantTM 800 (Cytvia 29399481). .. Antibodies that had higher than acceptable levels of LPS underwent LPS removal prior to injection using 10% Triton X-114 (Sigma-Aldrich X114).

    Article Title: Protocol for the Preparation of a Recombinant Treacle Fragment for Liquid–Liquid Phase Separation (LLPS) Assays
    Article Snippet: Nonidet P40 (NP40) (Thermo Scientific, catalog number: 85124); store at RT 21. .. One-Step Blue Protein Gel Stain (Biotium, catalog number: 21003); store at 4 °C 22. .. PageRuler prestained protein ladder (Thermo Scientific, catalog number: 26616); store at -20 °C 23.

    Article Title: Elucidation of Proteoforms of Chinese Hamster Ovary (CHO) Phospholipase B-Like 2 (PLBL2) Captured From a Monoclonal Antibody.
    Article Snippet: Precision Plus ProteinTM standard (1610373) was purchased from Bio‐Rad Laboratories (Hercules, CA, USA). .. One‐Step Blue® protein gel stain (21003‐1L) was from Biotium (Fremont, CA, USA). .. The hamster PLBL2 enzyme‐linked immunosorbent assay (ELISA) kit (MBS564202) was from MyBioSource (San Diego, CA, USA) and included the following components: antibody‐ coated ELISA micro plate, detection antibody (100× concentrate), HRP‐conjugated streptavidin (100× concentrate), calibrator, diluent solution, wash solution (20× concentrate), chromogen‐substrate solution (3,3′,5,5′‐ tetramethylbenzidine dihydrochloride, TMB), and stop solution (0.3M sulfuric acid).

    Article Title: The Yersinia pestis virulence effector YopM binds to a key regulatory site on the human pyrin death domain to inhibit inflammasome activation and effector-triggered immunity
    Article Snippet: The protein pellets were resuspended in 100 μl 1X sample buffer and 10 μl of the samples were run on 4–12% NuPAGE Bis-Tris SDS-PAGE gels (Invitrogen by ThermoFisher Scientific). .. The gels were stained using One-Step Blue Protein Gel Stain (Biotium) and imaged on an iBright FL1500 imager (ThermoFisher Scientific). .. THP-1 human monocyte cells (ATCC TIB-202) were grown in filter-sterilized RPMI-1640 media with 10% heat-inactivated fetal bovine serum (FBS), 0.05 mM β-MEand 1% pen-strep (10,000 U/mL Penicillin + 10,000 μg/mL streptomycin, Gibco) in 25 cm 2 flasks.

    Article Title: Structurally modified lysozyme via solvent Browning: Toward functional protein-based antioxidants.
    Article Snippet: Hen egg white lysozyme is a well-characterized protein extensively studied for its structural and functional properties.. Chemical modification strategies, including Maillard reaction, have been shown to alter lysozyme’s structure and bioactivity.. We previously identified a novel browning reaction initiated by simply dissolving proteins in a solvent mixture of dimethyl sulfoxide and acetone, resulting in timedependent chromophore formation.

    Article Title: Elucidation of Proteoforms of Chinese Hamster Ovary (CHO) Phospholipase B‐Like 2 (PLBL2) Captured From a Monoclonal Antibody
    Article Snippet: Precision Plus Protein TM standard (1610373) was purchased from Bio‐Rad Laboratories (Hercules, CA, USA). .. One‐Step Blue® protein gel stain (21003‐1L) was from Biotium (Fremont, CA, USA). .. The hamster PLBL2 enzyme‐linked immunosorbent assay (ELISA) kit (MBS564202) was from MyBioSource (San Diego, CA, USA) and included the following components: antibody‐coated ELISA micro plate, detection antibody (100× concentrate), HRP‐conjugated streptavidin (100× concentrate), calibrator, diluent solution, wash solution (20× concentrate), chromogen‐substrate solution (3,3′,5,5′‐ tetramethylbenzidine dihydrochloride, TMB), and stop solution (0.3 M sulfuric acid).

    Article Title: DCLRE1A orchestrates CAG repeat contraction following Cas12a-induced DNA breaks
    Article Snippet: The eluted protein was concentrated using Amicon Ultra 10K Centrifugal Filters (Millipore) with exchange to storage buffer: 20 mM HEPES (pH 7.9), 50 mM KCl, 10 mM MgCl 2 , 0.5 mM DTT, 0.05% Triton X-100, and 20% glycerol. .. The purity of the obtained protein fractions was estimated using SDS‒PAGE with One-Step Blue ® Protein Gel Stain (Biotium) and by Western blotting with anti-His-tag antibodies. ..

    Western Blot:

    Article Title: DCLRE1A orchestrates CAG repeat contraction following Cas12a-induced DNA breaks
    Article Snippet: The eluted protein was concentrated using Amicon Ultra 10K Centrifugal Filters (Millipore) with exchange to storage buffer: 20 mM HEPES (pH 7.9), 50 mM KCl, 10 mM MgCl 2 , 0.5 mM DTT, 0.05% Triton X-100, and 20% glycerol. .. The purity of the obtained protein fractions was estimated using SDS‒PAGE with One-Step Blue ® Protein Gel Stain (Biotium) and by Western blotting with anti-His-tag antibodies. ..



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